BlackNavara x Jaya High epicatechin Pool - SRA

SRX24037106: BlackNavara x Jaya High epicatechin Pool
1 ILLUMINA (Illumina NovaSeq 6000) run: 54.8M spots, 16.4G bases, 5.1Gb downloads

Design: Library preparation was performed using QIASeq FX DNA Library Preparation protocol. Briefly, 50 ng of Qubit quantified DNA was enzymatically fragmented, end-repaired and A-tailed in a one-tube reaction using the FX Enzyme Mix provided in the QIASeq FX DNAkit. The end-repaired and adenylated fragments were subjected to adapter ligation, whereby index-incorporated Illumina adapter was ligated, to generate sequencing library. The library was subjected to 6 cycles respectively of Indexing-PCR (Initial Denaturation at 98C for 2 min, cycling (98C for 20sec, 60C for 30sec, 72C for 30sec) and final extension at 72C for 1min) to enrich the adapter-tagged fragments. Finally, the amplified libraries were purified using HighPrep TM beads, followed by library quality control check.

Submitted by: Regional Agricultural Research Station, Kerala Agricultural University

Study: Mapping of nutraceutical traits in rice.

PRJNA1087694 • SRP497523 • All experiments • All runs

show Abstracthide Abstract

Identification of genomic regions/QTLs associated with nutraceutical traits using speciality rice.

Sample: F2:3 Bulk of 15 rice lines with high epicatechin pooled

SAMN40548731 • SRS20830512 • All experiments • All runs

Organism: Oryza sativa Indica Group

Library:

Name: SO_11602_Repl2_High Flavanoid Pooled

Instrument: Illumina NovaSeq 6000

Strategy: WGS